静岡大学農学部応用生命科学科
グローバル共創科学部
生物化学研究室
静岡大学農学部応用生命科学科
グローバル共創科学部
生物化学研究室

研究業績（教員別）

呉静 特任助教

1. 学術論文

2020以降

  1. Mori, T., Sugimoto, S., T Ishii, S, Wu, J., Nakamura, A., Dohra, H., Nagai, K., Kawagishi, H., and Hirai, H., Biotransformation and detoxification of tetrabromobisphenol A by white-rot fungus Phanerochaete sordida YK-624, J. Hazard. Mater., in press.
  2. Wu, J., Uchida, K., Yoshikawa, A., Hashimoto, M., Kondo, M., Nihei, K., Ishii, M., Choi, J-H., Miwa, Y., Shoda, C., Lee, D., Nakai, A., Kurihara, T., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C. Hiraia, H., and Kawagishia, H., “Fruiting Liquid” of Mushroom-Forming Fungi; Novel Source of Bioactive Compounds – Fruiting-Body Inducer, and HIF- and Axl- Inhibitor –, J. Agric. Food. Chem., 71, 13338−13345 (2023)
  3. Wu, J., Kamiishi, Y., Shimozaki, K., Kondo, M., Hashimoto, M., Choi, J-H., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C., Hirai, H., and Kawagishi H., Novel cyclopropyl compounds, cyclopropylvirgines A to G, from the fruiting bodies of Amanita virgineoides, Bull. Chem. Soc. Japan 12, 596–607 (2023)
  4. Wu, J., Ohura, T., Ogura, R., Wang, J., Choi, J-H., Kobori, H., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C., Takikawa, Y., H., Hirai, H., and Kawagishi H., Bioactive compounds from the mushroom-forming fungus Chlorophyllum molybdites, Antibiotics, 12(3), 596 (2023)
  5. Takemura, H., Choi, J-H., Fushimi, K., Narikawa, R., Kondo, M., Wu, J., Nelson, D. C., Suzuki, T., Ouchi, H., Inai, M., Hirai, H., and Kawagishi, H., Role of hypoxanthine-guanine phosphoribosyltransferase in the salvage pathway of fairy chemicals in rice, Org. Biomol. Chem., 21, 2556-2561 (2023)
  6. Kotajima, M., Choi, J-H., Suzuki, H., Suzuki, T., Wu, J., Hirai, H., Nelson, D. C., Ouchi, H., Inai, M., Dohra, H., and Kawagishi, H., Identification of biosynthetic and metabolic genes of 2-azahypoxanthine in Lepista sordida based on transcriptomic analysis, J. Nat. Prod., in press
  7. Kotajima, M., Choi, J-H., Suzuki, T., Wu, J., Hirai, H., Nelson, D. C., Ouchi, H., Inai, M., Dohra, H., and Kawagishi, H., The role of xanthine dioxygenase in the biosynthetic pathway of 2-aza-8-oxohypoxanthine of Lepista sordida, Biosci. Biotechnol. Biochem., 87(4), 420–425 (2023)
  8. Kotajima, M., Choi, J-H., Kondo, M., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E, C., Miwa Y., Shoda, S., Lee, D., Nakai, A., Kurihara, T., Wu, J., Hirai, H., and Kawagishi, H., Axl, immune checkpoint molecules and HIF inhibitors from the culture broth of Lepista luscina, Molecules, 227, 8925 (2022) 
  9. Kobori, H., Wu, J., Takemura, H., Choi, J-H., Tada, N., and Kawagish, H., Utilization of corn steep liquor for the production of fairy chemicals by Lepista sordida mycelia, J. Fungi, 8, 1269 (2022)
  10. Wang, J, Wu, J., Ogura, Y., Kobori,H., Choi, J-H, Hirai, H., Takikawa,Y., and Kawagishi, H., Anti-phytopathogenic bacterial fatty acids from the mycelia of the edible mushroom Agaricus blazei, Biosci. Biotechnol. Biochem., 86(10), 1327–1332 (2022)
  11. Jiang, Y., Wu, J., Kawagishi,H., Jiang, C., Zhou, G., Tong, Z., Tong , Y., and Wang, P., Study on secondary metabolites of endophytic fungus, Aspergillus fumigatus, from Crocus sativus L. guided by UHPLC-HRMS/MS-based molecular network, Int. J. Anal. Chem., Article ID 7067665, 1 (2022)
  12. Ito, A., Choi, J-H., Yokoyama-Maruyama, W., Kotajima, M., Wu, J., Suzuki, T., Terashima, Y., Suzuki, H., Hirai, H., Nelson, D. C., Tsunematsu. Y., Watanabe, K., Asakawa, T., Ouchi, H., Inai, M., Dohra, H, and Kawagishi H., 1,2,3-Triazine formation mechanism of a fairy chemical 2-azahypoxanthine in the fairy ring-forming fungus Lepista sordida, Org. Biomol. Chem.,  20, 2636-2642 (2022).
  13. Wang, J, Wu, J., Tsutsumi, K., Choi, J-H, Hirai, H., Kobori, H., Takikawa,Y., and Kawagishi, H., A new lanostane triterpenoid from the mushroom Hypholoma fasciculare, Biosci. Biotechnol. Biochem., 86(7), 819-823 (2022).
  14. Inoue, C., Yasuma, T., D'Alessandro-Gabazza, C. N., Toda, M., D'Alessandro, V. F., Inoue, R., Fujimoto, H., Kobori, H., Takeshita, A., Nishihama, K., Okano, Y., Wu, J., Kobayashi, T., Yano, Y., Kawagishi, H., and Gabazza, E. C., The fairy chemical imidazole-4-carboxamide inhibits the expression of Axl, PD-L1, and PD-L2 and improves response to cisplatin in cancer, Cells, 11, 374 (2022).
  15. Ouchi, H., Namiki, T., Iwamoto, K., Matsuzaki, N., Inai, M., Kotajima, M., Wu, J., Choi, J-H., Kimura, Y., Hirai,H., Xie, X., Kawagishi, H., and Kan,T., An S-adenosylhomocysteine analogue of a fairy chemical, imidazole-4-carboxamide, as its metabolite in rice and yeast, and synthetic investigations of related compounds, J. Nat. Prod., 84, 453−458 (2021).
  16. Lee, D., Miwa, Y., Wu, J., Shoda, C., Jeong, H., Kawagishi, H., Tsubota, K., and Kurihara. T., A fairy chemical suppresses retinal angiogenesis as a HIF inhibitor, Biomolecules, 10, 1405 (2020).
  17. Ito, A., Choi, J-H., Takemura,H., Kotajima,M., Wu, J., Tokuyama, S., Hirai, H., Asakawa, Ouchi, H., Inai, M., Kan, T., and Kawagishi, H., Biosynthesis of the fairy chemicals, 2-azahypoxanthine and imidazole-4-carboxamide, in the fairy ring-formingfungus Lepista sordida, J. Nat. Prod., 83,2469–2476 (2020).
  18. Aoshima, H., Hyodo, S., Ibuki, R., Wu, J., Choi, J-H., Kawagishi, H., Safety evaluation of 2-aza-8-oxohypoxanthine based on in vitro and human patch tests, Fundam. Toxicol. Sci., 7, 207-215 (2020).
  19. Oba, Y., Urai, M., Wu, J., Tomizawa, M., Kawagishi,H., and Hashimoto, K., Bitter compounds in two Tricholoma species, T. aestuans and T. virgatum, J. Antibiotic., 73, 697–701 (2020).
  20. Ridwan, Y. A., Wu, J., Harada, E., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma,T., Gabazza, E. C., Choi, J-H., Hirai, H., and Kawagishia, H., Axl and immune chechpoints inhibitors from fruiting bodies of Pleurocybella porrigens, J. Antibiotic., 73, 733–736 (2020).
  21. Malya, I. Y., Wu, J., Harada, E., Toda, M., D’Alessandro-Gabazza, C., Yasuma,T., Gabazza, E. C., Choi, J-H., Hirai, H., and Kawagishia, H., Plant growth regulators and axl and immune checkpoint inhibitors from the edible mushroom Leucopaxillus giganteus, Biosci. Biotechnol. Biochem., 80(7), 1332-1338 (2020).
  22. Niki, M., Hirata, Y., Nakazaki, A., Wu, J., Kawagishi, H., and Nishikawa, T., A biomimetic synthesis of chaxine and its related compounds, J. Org. Chem., 85(7), 4848–4860 (2020).
  23. D’Alessandro-Gabazza, C., Kobayashi, T., Yasuma, T., Toda, M., Kim, H., Fujimoto, H., Hataji, O., Takeshita, A., Nishihama, K., Okano, Y., Okano, Y., Nishii, Y., Tomaru, A., Fujiwara, K., D'Alessandro, V. F., Abdel-Hamid, A., Ren, Y., Pereira, G., Wright, C., Hernandez, A., Fields, C., Yau, P., Wang, S., Mizoguchi, A., Fukumura, M., Ohtsuka, J., Nosaka, T., Kataoka, K., Kondoh, Y., Wu, J., Kawagishi, H., Yano, Y., Mackie, R., Cann, I., and Gabazza, E. C., A Staphylococcus pro-apoptotic peptide induces acute exacerbation of pulmonary fibrosis, Nature Commun., 11, 1539 (2020).
  24. Nur, A.A.E., Ohshiro, T., Kobayashi, K., Wu, J., Wahyudin, E., Zhang, F., Hayashi, F., Kawagishi, H., and Tomoda, H., Inhibition of cholesteryl ester synthesis by polyacetylenes from Atractylodes rhizome, Bioorg. Med. Chem. Lett., 30, 126997 (2020).

2011-2019

  1. Choi, J-H., Matsuzaki,N., Wu, J., Kotajima, M., Hirai1, H., Kondo, M., Asakawa, T., Inai, M., Ouchi, H., Kan, T., and Kawagishi, H., Ribosides and ribotide of a fairy chemical, imidazole-4-carboxamide, as its metabolites in rice, Org. Lett., 21, 7841–7845 (2019).
  2. Liu, C., Minami, A., Ozaki, T., Wu, J., Kawagishi, H., Maruyama, J., and Oikawa, H., Efficient reconstitution of Basidiomycota diterpene erinacine gene cluster in Ascomycota host Aspergillus oryzae based on genomic DNA sequences, J. Am. Chem. Soc., 141, 15519−15523 (2019).
  3. Takemura, H., Choi, J-H., Matsuzaki, N., Taniguchi, Y., Wu, J., Hirai, H., Motohashi, R., Asakawa, T., Ikeuchi, K., Inai, M., Kan, T., and Kawagishi, H., A fairy chemical, imidazole-4-carboxamide, is produced on a novel purine metabolic pathway in rice, Sci. Rep., 9, 9899 (2019).
  4. Wu, J., Uchida, K., Ridwan, A., Kondo, M., Choi, J.-H., Hirai, H., and Kawagishi, H., Erinachromanes A and B and erinaphenol A from the culture broth of Hericium erinaceus , J. Agric. Food. Chem., 67, 3134-3139 (2019).
  5. Suzuki, T., Ono, A., Choi, J-H., Wu, J., Kawagishi, H., and Dohra, H., The complete mitochondrial genome sequence of the edible mushroom Stropharia rugosoannulata (Strophariaceae, Basidiomycota), Mitoch. DNA Part B: Res., 4(1), 570-572 (2019).
  6. Ridwan, Y. A.,Wu, J., Choi, J-H., Hirai, H., and Kawagishi, H., A novel plant growth regulator from Pholiota lubrica, Tetrahedron Lett., 59(26), 2559-2561(2018).
  7. Choi, J.-H.,Wu, J., Sawada, A., Takeda, S., Takemura, H., Yogosawa, K., Hirai, H., Kondo, M., Sugimoto, K., Asakawa, T., Inai, M., Kan, T., and Kawagishi, H., N-Glucosides of fairy chemicals, 2-azahypoxanthine and 2-aza-8-oxohypoxanthine, in rice. Org. Lett. , 20, 312-314 (2018).
  8. 崔 宰熏，呉 静，伏見圭司，平井浩文，河岸洋和，シバAgrostis stolonifera由来のコムラサキシメジ菌糸体に対する生育抑制物質，日本きのこ学会誌 ，25(4), 141–144 (2018).
  9. Arif, Y. R., Wu, J., Choi, J.-H., Hirai, H., and Kawagishi, H., Bioactive compounds from the edible mushroom Cortinarius caperatus, Mycoscience, 59, 172-175 (2018).
  10. Reddy, M. D., Kobori, H., Mori, T., Wu, J., Kawagishi, H., and Watkins, E. B., Gram-scale, stereoselective synthesis and biological evaluation of (+)-arimillariol C, J. Nat. Prod., 80, 2561–2565 (2017).
  11. Ito, A., Choi, J.-H., Wu, J., Tanaka, H., Hirai, H., and Kawagishi, H., Plant growth inhibitors from the culture broth of fairy ring-forming fungus Lepista sordida, Mycoscience, 58, 387–390 (2017).
  12. Ito, A., Choi, J.-H.,Wu, J., Ozawa, N., Hirai, H., and Kawagishi, H., Plant growth regulators from the edible mushroom Leccinum extremiorientale, Mycoscience, 58, 383–386 (2017).
  13. Qiu, W. T., Wu, J., Choi, J.-H., Hira, H., Nishida, H., and Kawagishi, H., Cytotoxic compounds against cancer cells from Bombyx mori inoculated with Cordyceps militaris, Biosci. Biotechnol. Biochem., 81, 1224–1226 (2017).
  14. Qiu W. T., Kobori H.,Wu, J., Choi J.-H., Hirai H., and Kawagishi H., Plant growth regulators from the fruiting bodies of Tricholoma flavovirens. Biosci. Biotechnol. Biochem.81(3), 441-444 (2017).
  15. Matsuzaki N., Wu, J., Kawaide M., Choi J.-H., Hirai H., and Kawagishi H., Plant growth regulatory compounds from the mushroom Russula vinosa. Mycoscience, 57, 404-407 (2016).
  16. Wu, J., Tokunaga, T., Kondo, M., Ishigami, K., Tokuyama, S., Suzuki, T., Choi, J.-H., Hirai, H., and Kawagishi, H., Erinaceolactones A to C, from the culture broth of Hericium erinaceus. J. Nat. Prod., 78, 155-158 (2015).
  17. Wu J., Suzuki T., Choi J.-H., Yasuda N., Noguchi K., Hirai H., and Kawagishi H., An unusual sterol from the mushroom Stropharia rugosoannulata. Tetrahedron Lett., 54, 4900-4902 (2013).
  18. Wu J., Kobori H., Kawaide M., Suzuki T., Choi J.-H., Yasuda N., Noguchi K., Matsumoto T., Hirai H., and Kawagishi H., Isolation of bioactive steroids from the mushroom Stropharia rugosoannulata and absolute configuration of strophasterol B. Biosci. Biotechnol. Biochem., 77 (8), 1779-1781 (2013).
  19. Wu J., Tokuyama S., Nagai K., Yasuda N., Noguchi K., Matsumoto T., Hirai H., and Kawagishi H., Strophasterols A to D with the unprecedented steroid skeleton, from the mushroom Stropharia rugosoannulata. Angew. Chem. Int. Ed., 51, 10820-10822 (2012).
  20. Wu J., Tsujimori M., Hirai H., and Kawagishi H., Novel compounds from the mycelia and fruiting bodies of Climacodon septentrionalis. Biosci. Biotechnol. Biochem., 75 (4), 783-785 (2011).
  21. Wu J., Choi J-H., Yoshida M., Hirai H., Harada E., Masuda K., Koyama T., Yazawa K., Noguchi K., Nagasawa K., and Kawagishi H., Osteoclast-forming suppressing compounds, gargalols A, B and C, from the edible mushroom Grifola gargal. Tetrahedron, 67, 6576-6581 (2011).
  22. Wu J., Fushimi K., Tokuyama S., Ohno M., Miwa T., Koyama T., Yazawa K., Nagai K., Matsumoto T., Hirai H., and Kawagishi H., Functional-food constituents in the fruiting bodies of Stropharia rugosoannulata. Biosci. Biotechnol. Biochem., 75 (8), 1631-1634 (2011).

2. 総説

  1. Wu, J. and Kawagishi, H., Plant growth regulators from mushrooms, J. Antibiotic., 73, 657–665 (2020).
  2. 呉静，河岸洋和，キノコ由来の生物活性２次代謝産物に関する化学的研究，化学と生物，58, 231–239 (2020).

3. 著書

  1. 呉静，清水邦義，第8章　ヤマブシタケ，「きのこの生物活性と応用展開」，河岸洋和（監修），シーエムシー出版，pp.155-167, (2021)，全359頁

4. 受賞

  1. 日本農芸化学会、農芸化学奨励賞 (2024)
    「高等菌類由来の生物活性物質に関する化学的研究」
  2. 第62回天然有機化合物討論会奨励賞 (2020)
    「高等菌類におけるホルモンの探索」
  3. 日本農芸化学若手女性研究者賞（2019）
    「キノコ由来の生物活性2次代謝産物に関する化学的研究」
  4. 日本農芸化学会 2016年度大会トピックス賞(2016)
    「高等菌類の子実体形成物質の探索」
  5. 日本農芸化学会 2014年度大会トピックス賞 (2014)
    「高等菌類の子実体形成物質の探索」
  6. 第12回新規素材探索研究会　最優秀ポスター賞 (2013)
    「菌類の子実体形成物質の探索」
  7. China-Korea-Japan Partnership in Science and Technology　Best Poster Award (2013)
    「Strophasterols A to D with an Unprecedented Steroid Skeleton: From the Mushroom Stropharia rugosoannulata」
  8. シーズ＆ニーズビジネスマッチング研究発表会　最優秀ポスター賞 (2012)
    「キノコからの新規ステロイド骨格化合物の発見およびその機能性」
  9. The 16th Shizuoka Forum on Health and Longevity　Poster Presentation Award (2011)
    「Functional-food constituents in Stropharia rugosoannulata」
  10. Convergence Technology of Bio and Nano Science　Best Poster Award (2011)
    「Functional-food constituents in Stropharia rugosoannulata」

研究業績（教員別）