Biosci. Biotechnol. Biochem. 85, 630-633 (2021).
An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens
Suzuki, T., Nakamura, L., Inayoshi, S., Tezuka, Y., Choi, J-H., Dohra, H., Sasanami, T. Hirai, H., and Kawagishi H.
In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.