Molecules 227, 8925 (2022)
Axl, immune checkpoint molecules and HIF inhibitors from the culture broth of Lepista luscina
Kotajima, M., Choi, J-H., Kondo, M., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E, C., Miwa Y., Shoda, S., Lee, D., Nakai, A., Kurihara, T., Wu, J., Hirai, H., and Kawagishi, H.
Two compounds 1 and 2 were isolated from the culture broth of Lepista luscina. This is the first time that compound 1 was isolated from a natural source. The structure of compound 1 was identified via 1D and 2D NMR and HRESIMS data. Compounds 1 and 2 along with 8-nitrotryptanthrin (4) were evaluated for their biological activities using the A549 lung cancer cell line. As a result, 1 and 2 inhibited the expression of Axl and immune checkpoint molecules. In addition, compounds 1, 2 and 4 were tested for HIF inhibitory activity. Compound 2 demonstrated statistically significant HIF inhibitory effects on NIH3T3 cells and 1 and 2 against ARPE19 cells.