研究業績

2026年

学術論文

• "Plant growth regulators from the fruiting bodies and scrap cultivation beds of Hypsizygus marmoreus"
  Sunuwar, A., Wu, J., Choi, J-H., Hirai, H., and Kawagishi. H.
  Int. J. Med. Mushr. 28, 75-82 (2026).

  • DOI: 10.1615/IntJMedMushrooms.2025060926

2025年

学術論文

• "Comprehensive transcriptomic analysis revealing that 2-azahypoxanthine treatment induces the stress response in Arabidopsis thaliana"
  Tanaka, Y., Suzuki, T., Mitsukuni, K., Choi, J-H., Nomura, T., Xie, X., Kojima, M., Takebayashi, Y., Sakakibara, H., Ouchi, H., Inai, M., Dohra, H., Okamoto, M., and Kawagishi, H.
  Biosci. Biotechnol. Biochem. 90, 27–36 (2025).

  • DOI: 10.1093/bbb/zbaf162
• "New metabolites of fairy chemicals in tea and coffee, methylated AHX and AOH"
  Abe, T., Ino, S., Choi, -H., Wu, J., Jing Wu, Yokobori, S., Ouchi, H., Takita, R., Amaike, K., Itami, K., Sato, M., Watanabe K., Hirai, H., and Kawagishi. H.
  J. Agric. Food. Chem. 73, 28802−28810 (2025).

  • DOI: 10.1021/acs.jafc.5c05834
• "Transcriptomic resource and hormonal profiling of the smut fungus, Ustilago esculenta, coexisting with Zizania latifolia and forming Makomotake"
  Tanaka, Y., Ono, A., Aoki, D., Matsuda, R., Kitsukawa, Y., Choi, J-H., Xie, X., Aime, M. C., Kawagishi, H., Suzuki, T.
  Biosci. Biotechnol. Biochem. 89, 1293–1301 (2025).

  • DOI: 10.1093/bbb/zbaf084
• "Construction of a comprehensive functional screening system for Phanerochaete sordida YK-624 cytochrome P450s: identification of catalytic enzymes for emerging contaminant degradation"
  R. Yin, Y. Nayuki, W. Park, R. Matsuura, C. Otomaru, H. Yamada, A. Ono, H. Ichinose, T. Mori, H. Kawagishi, H. Hirai
  Journal of Hazardous Materials 489, 137666 (2025).

  • DOI: 10.1016/j.jhazmat.2025.137666
• "Insights into the imidaclothiz biodegradation by the white-rot fungus Phanerochaete sordida YK-624 under ligninolytic conditions"
  R. Yin, M. Chang, R. Ma, J. Wang, N. Wang, T. Xiao, H. Hirai
  Journal of Agricultural and Food Chemistry 73, 12877-12886 (2025).
• "Plant growth regulators from the liquid culture of Lepista sordida"
  A. Sunuwar, J-H. Choi, M. Sumida, K. Kume, J. Wu, A. Ito, K. Yamashita, H. Hirai, H. Kawagishi
  Bioscience, Biotechnology, and Biochemistry 89, 934-940 (2025).

  • DOI: 10.1093/bbb/zbaf048
• "Uncovering hericenones from the fruiting bodies of Hericium erinaceus through interdisciplinary collaboration"
  Wang, J, Wu, J., Yamaguchi, Y., Nagai, K., Liu, C., Choi, J-H, Hirai, H., Xie, X., Kobayashi, S., and Kawagishi, H.
  (2025).

  • DOI: 10.1021/acs.jnatprod.4c01018
• "Construction of a comprehensive functional screening system for Phanerochaete sordida YK-624 cytochrome P450s: identification of catalytic enzymes for emerging contaminant degradation"
  R. Yin, Y. Nayuki, W. Park, R. Matsuura, C. Otomaru, H. Yamada, A. Ono, H. Ichinose, T. Mori, H. Kawagishi, H. Hirai
  Journal of Hazardous Materials 489, 137666 (2025).

  • DOI: 10.1016/j.jhazmat.2025.137666

2024年

学術論文

• "Biodegradation of non-steroidal anti-inflammatory drug loxoprofen by a hyper lignin-degrading fungus Phanerochaete sordida YK-624 under non-ligninolytic conditions"
  Yin, R., Wu, J., Nagai, K., Mori, T., Ono,A., Wang,J., Kawagishi, H.,and Hirai, H.
  Chemosphere 364, 43265 (2024).

  • DOI: 10.1016/j.chemosphere.2024.143265
• "Biotransformation and detoxification of tetrabromobisphenol A by white-rot fungus Phanerochaete sordida YK-624"
  Mori, T., Sugimoto, S., T Ishii, S, Wu, J., Nakamura, A., Dohra, H., Nagai, K., Kawagishi, H., and Hirai, H.
  J. Hazard. Mater. 465133469 (2024).

  • DOI: 10.1016/j.jhazmat.2024.133469

2023年

学術論文

• "Ergosterol and its metabolite induce ligninolytic activity in lignin degrading fungus Phanerochaete sordida YK-624"
  J. Wang, R. Yin, Y. Hashizume, Y. Todoroki, T. Mori, H. Kawagishi, H. Hirai
  Journal of Fungi 9, 951 (2023).

  • DOI: 10.3390/jof9090951
• " “Fruiting Liquid” of Mushroom-Forming Fungi; Novel Source of Bioactive Compounds – Fruiting-Body Inducer, and HIF- and Axl- Inhibitor –"
  Wu, J., Uchida, K., Yoshikawa, A., Hashimoto, M., Kondo, M., Nihei, K., Ishii, M., Choi, J-H., Miwa, Y., Shoda, C., Lee, D., Nakai, A., Kurihara, T., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C. Hirai, H., and Kawagishi, H.
  J. Agric. Food. Chem. 71, 13338-13345 (2023).

  • DOI: 10.1021/acs.jafc.3c03633
• "Aerobic H2production related to formate metabolism in white-rot fungi"
  T. Mori, S. Takahashi, A. Soga, M. Arimoto, R. Kishikawa, Y. Yama, H. Dohra, H. Kawagishi, H. Hirai
  Frontiers in Fungal Biology 4, 1201889 (2023).

  • DOI: 10.3389/ffunb.2023.1201889
• "Construction of white-rot fungal–bacterial consortia with improved ligninolytic properties and stable bacterial community structure"
  T. Mori, T. Terashima, M. Matsumura, K. Tsuruta, H. Dohra, H. Kawagishi, H. Hirai
  ISME Communications, 3, 61 (2023).

  • DOI: 10.1038/s43705-023-00270-4
• " Effects on microstructures of decayed wood particles in spent mushroom substrates on the enzymatic saccharification and fermentation properties"
  T. Mori, S. Higashi, H. Kimura, H. Kawagishi, H. Hirai
  Bioresource Technology Reports 22, 101494 (2023).

  • DOI: 10.1016/j.biteb.2023.101494
• "Novel cyclopropyl compounds, cyclopropylvirgines A to G, from the fruiting bodies of Amanita virgineoides"
  Wu, J., Kamiishi, Y., Shimozaki, K., Kondo, M., Hashimoto, M., Choi, J-H., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C., Hirai, H., and Kawagishi H.
  Bull. Chem. Soc. Japan 12, 596-607 (2023).

  • DOI: 10.1246/bcsj.20230036
• "Bioactive compounds from the mushroom-forming fungus Chlorophyllum molybdites"
  Wu, J., Ohura, T., Ogura, R., Wang, J., Choi, J-H., Kobori, H., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E. C., Takikawa, Y., H., Hirai, H., and Kawagishi H.,
  Antibiotics 12, 596 (2023).

  • DOI: 10.3390/antibiotics12030596
• " Role of hypoxanthine-guanine phosphoribosyltransferase in the salvage pathway of fairy chemicals in rice"
  Takemura, H., Choi, J-H., Fushimi, K., Narikawa, R., Kondo, M., Wu, J., Nelson, D. C., Suzuki, T., Ouchi, H., Inai, M., Hirai, H., and Kawagishi, H.
  Org. Biomol. Chem. 21, 2556-2561 (2023).

  • DOI: 10.1039/D3OB00026E
• "Identification of biosynthetic and metabolic genes of 2-azahypoxanthine in Lepista sordida based on transcriptomic analysis"
  Kotajima, M., Choi, J-H., Suzuki, H., Suzuki, T., Wu, J., Hirai, H., Nelson, D. C., Ouchi, H., Inai, M., Dohra, H., and Kawagishi, H.
  J. Nat. Prod. 86, 710–718 (2023).

  • DOI: 10.1021/acs.jnatprod.2c00789
• "The role of xanthine dioxygenase in the biosynthetic pathway of 2-aza-8-oxohypoxanthine of Lepista sordida"
  Kotajima, M., Choi, J-H., Suzuki, T., Wu, J., Hirai, H., Nelson, D. C., Ouchi, H., Inai, M., Dohra, H., and Kawagishi, H.
  Biosci. Biotechnol. Biochem. 87 , 420–425 (2023).

  • DOI: 10.1093/bbb/zbad005
• "Possible molecular mechanism for acute encephalopathy by angel-wing mushroom ingestion -involvement of three constituents in onset-"
  Suzuki, T., Asakawa, T., Maekawa, F., Kimura, E., Tezuka, Y., Nakamura, L., Sato, T., Arai, Y., Choi, J-H., Suzuki, M., Dohra, H., Hirai, H., and Kawagishi H.
  Toxicon 221, 106958 (2023).

  • DOI: 10.1016/j.toxicon.2022.106958

2022年

学術論文

• "Axl, immune checkpoint molecules and HIF inhibitors from the culture broth of Lepista luscina"
  Kotajima, M., Choi, J-H., Kondo, M., D’Alessandro-Gabazza, C. N., Toda, M., Yasuma, T., Gabazza, E, C., Miwa Y., Shoda, S., Lee, D., Nakai, A., Kurihara, T., Wu, J., Hirai, H., and Kawagishi, H.
  Molecules 227, 8925 (2022).

  • DOI: 10.3390/molecules27248925
• "Utilization of corn steep liquor for the production of fairy chemicals by Lepista sordida mycelia "
  Kobori, H., Wu, J., Takemura, H., Choi, J-H., Tada, N., and Kawagish, H.
  J. Fungi 8, 12, 1269 (2022).

  • DOI: 10.3390/jof8121269
• "The complete mitochondrial genome of the white-rot fungus Phanerochaete sordida YK-624"
  Mori, T., Dohra, H., Kawagishi, H., and Hirai, H.
  Mitoch. DNA Part B: Res. 7, 9, 1743-1745 (2022).

  • DOI: 10.1080/23802359.2022.2124830
• "Biotransformation of bisphenol F by white-rot fungus Phanerochaete sordida YK-624 under non-ligninolytic condition, Applied Microbiology and Biotechnology"
  R. Yin, X. Zhang, B. Wang, J. Jia, N. Wang, C. Xie, P. Su, P. Xiao, J. Wang, T. Xiao, B. Yan, H. Hirai
  (2022).

  • DOI: 10.1007/s00253-022-12133-4
• "CmLec4, a lectin from the fungus Cordyceps militaris, controls host infection and fruiting body formation"
  Ono, A., Suzuki,T., Takeshima, Y., Kashiwa, T., Motoyama, T., Choi, J-H., Sato, C., Konno, N., Miyakawa, H., Ogata, M., Hirai, H., Dohra, H., Osada, H., and Kawagishi, H.
  Int. J. Biol. Macromol. 215, 303-311 (2022).

  • DOI: 10.1016/j.ijbiomac.2022.06.106
• "Anti-phytopathogenic bacterial fatty acids from the mycelia of the edible mushroom Agaricus blazei"
  Wang, J, Wu, J., Ogura, Y., Kobori,H., Choi, J-H, Hirai, H., Takikawa,Y., and Kawagishi, H.
  Biosci. Biotechnol. Biochem. 86, 10, 1327–1332 (2022).

  • DOI: 10.1093/bbb/zbac117
• "Meta-analysis of neonicotinoid insecticides in global surface waters"
  J. Wang, R. Yin, Y. Liu, B. Wang, N. Wang, P. Xiao, T. Xiao, H. Hirai
  Environmental Science and Pollution Research 30, 1039-1047 (2022).
• "A new lanostane triterpenoid from the mushroom Hypholoma fasciculare"
  Wang, J, Wu, J., Tsutsumi, K., Choi, J-H, Hirai, H., Kobori, H.,Takikawa,Y., and Kawagishi, H.
  Biosci. Biotechnol. Biochem. 86, 819-823 (2022).

  • DOI: 10.1093/bbb/zbac050
• "The complete mitochondrial genome sequence of the fairy ring-forming fungus Lepista sordida"
  Choi, J-H, Suzuki, T., Ono, A., Kotajima, M., Tanaka, Y., Suzuki, T., Kawagishi, H., and Dohra, H.
  Mitoch. DNA Part B: Res. 7, 4, 712–714 (2022).

  • DOI: 10.1080/23802359.2022.2067496
• "Potential of fairy chemicals as functional cosmetic ingredients: effect of 2-aza-8-oxohypoxanthine (AOH) on skin lightness"
  Aoshima, H., Ito, M., Ibuki, R., and Kawagishi, H.
  Int. J. Med. Mushr. 24, 41–47 (2022).
• "1,2,3-Triazine formation mechanism of a fairy chemical 2-azahypoxanthine in the fairy ring-forming fungus Lepista sordida"
  Ito, A., Choi, J-H., Yokoyama-Maruyama, W., Kotajima, M., Wu, J., Suzuki, T., Terashima, Y., Suzuki, H., Hirai, H., Nelson, D. C., Tsunematsu. Y., Watanabe, K., Asakawa, T., Ouchi, H., Inai, M., Dohra, H, and Kawagishi H.
  Org. Biomol. Chem. 20, 2636-2642 (2022).

  • DOI: 10.1039/d2ob00328g
• " RNA-Seq analysis of Phanerochaete sordida YK-624 degrades neonicotinoid pesticide acetamiprid, Environmental Technology"
  J. Wang, Y. Liu, R. Yin, N. Wang, T. Xiao, H. Hirai
  Environmental Technology 20, 1-8 (2022).

  • DOI: 10.1080/09593330.2022.2026488
• "The fairy chemical imidazole-4-carboxamide inhibits the expression of Axl, PD-L1, and PD-L2 and improves response to cisplatin in cancer"
  Inoue, C., Yasuma, T., D'Alessandro-Gabazza, C. N., Toda, M., D'Alessandro, V. F., Inoue, R., Fujimoto, H., Kobori, H., Takeshita, A., Nishihama, K., Okano, Y., Wu, J., Kobayashi, T., Yano, Y., Kawagishi, H., and Gabazza, E. C.
  Cells 11, 347 (2022).

  • DOI: 10.3390/cells11030374

2021年

学術論文

• "Transcriptomic analysis reveals ligninolytic enzymes of white-rot fungus Phanerochaete sordida YK-624 participating in bisphenol F biodegradation under ligninolytic conditions"
  Wang, J., Yin, R., Zhang, X., Wang, N., Xiao, P., Hirai, H., Xiao, T.
  Environ. Sci. Pollut. Res. Int. 28, 62390-62397 (2021).

  • DOI: 10.1007/s11356-021-15012-z
• "Ethanol fermentation by saprotrophic white-rot fungus Phanerochaete sordida YK-624 during wood decay as a system for short-term resistance to hypoxic conditions"
  Mori, T., Masuda, A., Kawagishi, H., Hirai, H.
  J. Biosci. Bioeng. 133, 64-69 (2021).

  • DOI: 10.1016/j.jbiosc.2021.10.002
• "Draft genome sequence of the white-rot fungus Phanerochaete sordida YK-624"
  Mori, T., Dohra, H., Suzuki, T., Kawagishi, H., Hirai, H.
  Microbiology Resource Announcements 42, e00842-21 (2021).

  • DOI: 10.1128/MRA.00842-21
• "Transcriptomics analysis reveals the high biodegradation efficiency of white-rot fungus Phanerochaete sordida YK-624 on native lignin"
  Wang, J., Suzuki, T., Yin, R., Dohra, H., Mori, T., Kawagishi, H., and Hirai, H.
  J. Biosci. Bioeng. 132, 253-257 (2021).

  • DOI: 10.1016/j.jbiosc.2021.05.009
• "Clinical evaluation of topical lotion containing 2-aza-8-oxohypoxanthine on skin barrier function against water loss"
  Aoshima, H., Ibuki, R. H., Ito, M., and Kawagishi, H.
  Cosmetics 8, 83 (2021).

  • DOI: 10.3390/cosmetics8030083
• "Improvement of saccharide yield from wood by simultaneous enzymatic delignification and saccharification using a ligninolytic enzyme and cellulase"
  Mori, T., Ikeda, K., Kawagishi, H., Hirai, H.
  J. Biosci. Bioeng. 132, 213-219 (2021).

  • DOI: 10.1016/j.jbiosc.2021.04.016
• "Safety evaluation of 2-aza-8-oxohypoxanthine by in vitro skin sensitization and human tests"
  Aoshima, H., Matsumoto, T., Ibuki, R. and Kawagishi, H.
  Fundam. Toxicol. Sci. 8, 123-133 (2021).

  • DOI: 10.2131/fts.8.123
• "The potential of 2-aza-8-oxohypoxanthine as a cosmetic ingredient"
  Aoshima, H., Ito, M., Ibuki, R. and Kawagishi, H.
  Cosmetics 8, 60 (2021).

  • DOI: 10.3390/cosmetics8030060
• "Quantification of ustalic acid, a chemotaxonomic marker, in Tricholoma ustale using liquid chromatography–mass spectrometry"
  Ito,T., Nagai, H., Aoki, W., Yamada, A., Kawagishi, H., Fukaya, M., and Konishi, H.
  J. Nat. Med. 75, 688-691 (2021).

  • DOI: 10.1007/s11418-021-01496-z.
• "An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens"
  Suzuki, T., Nakamura, L., Inayoshi, S., Tezuka, Y., Choi, J-H., Dohra, H., Sasanami, T. Hirai, H., and Kawagishi H.
  Biosci. Biotechnol. Biochem. 85, 630-633 (2021).

  • DOI: 10.1093/bbb/zbaa058
• "S-Adenosylhomocysteine Analogue of a Fairy Chemical, Imidazole-4-carboxamide, as its Metabolite in Rice and Yeast and Synthetic Investigations of Related Compounds"
  Ouchi, H., Namiki, T., Iwamoto, K., Matsuzaki, N., Inai, M., Kotajima, M., Wu, J., Choi, J-H., Kimura, Y., Hirai,H., Xie, X., Kawagishi, H., and Kan,T
  J. Nat. Prod. 84, 453-458 (2021).

  • DOI: 10.1021/acs.jnatprod.0c01269
• "White-rot fungus Phanerochaete chrysosporium metabolizes chloropyridinyl-type neonicotinoid insecticides by an N-dealkylation reaction catalyzed by two cytochrome P450s"
  T. Mori, H. Ohno, H. Ichinose, H. Kawagishi, H. Hirai
  Journal of Hazardous Materials 402, 123831 (2021).

  • DOI: 10.1016/j.jhazmat.2020.123831

2020年

学術論文

• "A fairy chemical suppresses retinal angiogenesis as a HIF inhibitor"
  Lee, D., Miwa, Y., Wu, J., Shoda, C., Jeong, H., Kawagishi, H., Tsubota, K., and Kurihara. T.
  Biomolecules 10, 1405 (2020).

  • DOI: 10.3390/biom10101405
• "木質バイオマスからのメタン発酵前処理に適した白色腐朽菌の選抜"
  森智夫，岩田実子，木村浩之，河岸和洋，平井浩文
  日本きのこ学会誌 28, 2, 56-61 (2020).
• "Biosynthesis of the fairy chemicals, 2-azahypoxanthine and imidazole-4-carboxamide, in the fairy ring-formingfungus Lepista sordida"
  Ito, A., Choi, J-H., Takemura,H., Kotajima,M., Wu, J., Tokuyama, S., Hirai, H., Asakawa, Ouchi, H., Inai, M., Kan, T., and Kawagishi, H.
  J. Nat. Prod. 83, 2469－2476 (2020).

  • DOI: 10.1021/acs.jnatprod.0c00394
• "Bitter compounds in two Tricholoma species, T. aestuans and T. virgatum"
  Oba, Y., Urai, M., Wu, J., Tomizawa, M., Kawagishi,H., and Hashimoto, K.
  J. Antibiotic. 73, 697-701 (2020).
• "Safety evaluation of 2-aza-8-oxohypoxanthine based on in vitro and human patch tests"
  Aoshima, H., Hyodo, S., Ibuki, R., Wu, J., Choi, J-H., Kawagishi, H.
  Fundam. Toxicol. Sci. 7, 5, 207-215 (2020).

  • DOI: 10.2131/fts.7.207
• "Axl and immune checkpoints inhibitors from the fruiting bodies of Pleurocybella porrigens"
  Ridwan, Y. A., Wu, J., Harada, E., D’Alessandro-Gabazza, C., Toda, M., Yasuma,T., Gabazza, E. C., Choi, J-H., Hirai, H., and Kawagishi, H.
  J. Antibiotic. 73, 733-736 (2020).
• "Effects of imidazole-4-carboxamide and 2-azahypoxanthine on the growth of Pinus densiflora seedlings and its ectomycorrhizal formation with Tricholoma matsutake"
  Nakano, S., Yamanaka, T., and Kawagishi, H.
  Mycoscience 61, 5, 259-263 (2020).

  • DOI: 10.1016/j.myc.2020.04.004
• "A Staphylococcus pro-apoptotic peptide induces acute exacerbation of pulmonary fibrosis"
  D’Alessandro-Gabazza, C., Kobayashi, T., Yasuma, T., Toda, M., Kim, H., Fujimoto, H., Hataji, O., Takeshita, A., Nishihama, K., Okano, Y., Okano, Y., Nishii, Y., Tomaru, A., Fujiwara, K., D'Alessandro, V. F., Abdel-Hamid, A., Ren, Y., Pereira, G., Wright, C., Hernandez, A., Fields, C., Yau, P., Wang, S., Mizoguchi, A., Fukumura, M., Ohtsuka, J., Nosaka, T., Kataoka, K., Kondoh, Y., Wu, J., Kawagishi, H., Yano, Y., Mackie, R., Cann, I., and Gabazza, E. C.
  Nature Commun 11, 1539 (2020).
• "Plant growth regulators and axl and immune checkpoint inhibitors from the edible mushroom Leucopaxillus giganteus"
  Malya, I. Y., Wu, J., Harada, E., Toda, M., D’Alessandro-Gabazza, C., Yasuma,T., Gabazza, E. C., Choi, J-H., Hirai, H., and Kawagishi, H.
  Biosci. Biotechnol. Biochem. 80, 7, 1332-1338 (2020).

  • DOI: 10.1080/09168451.2020.1743170
• "A biomimetic synthesis of chaxine and its related compounds"
  Niki, M., Hirata, Y., Nakazaki, A., Wu, J., Kawagishi, H., and Nishikawa, T.
  J. Org. Chem. 85, 7, 4848-4860 (2020).

  • DOI: 10.1021/acs.joc.9b03482
• "Self-fusion and fusion cell isolation of transformants derived from white rot fungus Phanerochaete sordida YK-624 by simple visual method"
  Mori, T., Kondo, O., Sumiya, T., Kawagishi, H., and Hirai, H.
  J. Bioscie. Bioeng. 129, 146-149 (2020).

  • DOI: 10.1016/j.jbiosc.2019.08.011
• "Inhibition of cholesteryl ester synthesis by polyacetylenes from Atractylodes rhizome"
  Nur, A.A.E., Ohshiro, T., Kobayashi, K., Wu, J., Wahyudin, E., Zhang, F., Hayashi, F., Kawagishi, H., and Tomoda, H.
  Bioorg. Med. Chem. Lett. 30, 126997 (2020).

  • DOI: 10.1016/j.bmcl.2020.126997
• "Medicinal mushroom, Grifola gargal (Agaricomycetes), lowers triglyceride in animal models of obesity and diabetes and in adults with prediabetes"
  Harada, E., Morizono, T., Kanno, Saito, M., and Kawagishi, H.
  Int. J. Med. Mushr. 22, 1, 79-91 (2020).

  • DOI: 10.1615/IntJMedMushrooms.2019033285